Chapter 4 - Functional connectivity of the dorsal cortex at rest
This chapter examines whether the reduction in MeCP2 expression affects the flow of information across the dorsal cortex at rest. Supplementary material comprises of interactive heatmaps and graph models of functional connectivity at rest, as well as videos of grooming and quiescent behaviour.
Supplementary Figure 4.1.: Example of behavioural quiescence in a Wt mouse
Example video of “typical” quiescent behaviour in a Wt mouse. The mouse is sat fairly still in the head-restrain apparatus, not really doing a whole lot.
Supplementary Figure 4.2.: Example of a grooming bout in a Wt mouse
Example video of “typical” grooming in a Wt mouse. The video shows a mouse grooming its face and head while head-restrained.
Supplementary Figure 4.3.: Map of interareal functional connectivity in Wt mice at 16 weeks
Interactive heatmap of resting-state functional connectivity in 16-week old Wt mice (N=4). Each square represents the Pearson’s correlation coefficient r for a pair of cortical areas. Hovering above a square in this plot will reveal the full name of the areas in the pair (as defined in the Allen Brain Atlas) and the associated r value.
Supplementary Figure 4.4.: Map of interareal functional connectivity in Mecp2+/- mice at 16 weeks
Similar to the above. Interactive heatmap of resting-state functional connectivity in 16-week old Mecp2+/- mice (N=4). Each square represents the Pearson’s correlation coefficient r for a pair of cortical areas. Hovering above a square in this plot will reveal the full name of the areas in the pair (as defined in the Allen Brain Atlas) and the associated r value.
Supplementary Figure 4.5.: Map of interareal functional connectivity differences between Wt and Mecp2+/- mice at 16 weeks
Interactive heatmap of the difference in resting-state functional connectivity between 16-week old Wt and Mecp2+/- mice (N=4). Each square represents the difference in Pearson’s correlation coefficient ∆r for a pair of cortical areas between the two genotypes (Wt - Mecp2+/-). Negative values indicate that the correlation is higher in Wt mice (i.e. hypoconnectivity in Mecp2+/-), while positive values indicate that the correlation is higher in Mecp2+/- mice. Hovering above a square in this plot will reveal the full name of the areas in the pair, the ∆r value with its confidence interval in square brackets and the associated p-value.
Supplementary Figure 4.6.: Graph projection of the functional resting state networks in 16 week old Wt and Mecp2+/- mice
Interactive graph projection of the resting state functional connectivity networks of 16 week old Wt and Mecp2+/- mice. Each node (circles) represents a cortical area, while edges (lines) represent significant (direct) functional connections between pairs of nodes. Hovering above a node will reveal its full name and the region it belongs to (visual, motor, somatosensory or retrosplenial), while clicking a node will visually isolate the direct network connections it is involved in (click the blank space to reset).
Wt resting state network (16 weeks)
Mecp2+/- resting state network (16 weeks)
Supplementary Figure 4.7.: Topographical differences between Wt and Mecp2+/- resting state networks at 16 weeks
Interactive map of topographical differences between Wt and Mecp2+/- resting state networks. Each square in this map represents a functional connection between two areas. Blue squares indicate connections present only in the modelled networks of Wt mice (i.e. absent in Mecp2+/-), while red squares indicate connections found only in Mecp2+/- networks. White squares indicate no difference. Hovering above a square in this plot will reveal the full name of the areas in the pair.
Supplementary Figure 4.8.: Map of interareal functional connectivity differences between Wt and Mecp2+/- mice at 16, 20 and 24 weeks
Interactive heatmap of the difference in resting-state functional connectivity Wt and Mecp2+/- mice (N=4) at 16, 20 and 24 weeks. Use the slider on the right hand side of the plot to switch between different age points. Each square represents the difference in Pearson’s correlation coefficient ∆r for a pair of cortical areas between the two genotypes (Wt - Mecp2+/-). Negative values indicate that the correlation is higher in Wt mice (i.e. hypoconnectivity in Mecp2+/-), while positive values indicate that the correlation is higher in Mecp2+/- mice. Hovering above a square in this plot will reveal the full name of the areas in the pair, the ∆r value with its confidence interval in square brackets and the associated p-value.
Supplementary Figure 4.9.: Graph models for Wt and Mecp2+/- mice at 16, 20 and 24 weeks
Interactive graph projection of the resting state functional connectivity networks of old Wt and Mecp2+/- mice at 16, 20 and 24 weeks. Using the Behaviour slider on the right hand side of the plot to switch between different age points, while the Genotype toggle can be used to select which genotype to display. Each node (circles) represents a cortical area, while edges (lines) represent significant (direct) functional connections between pairs of nodes. Hovering above a node will reveal its full name and the region it belongs to (visual, motor, somatosensory or retrosplenial), while clicking a node will visually isolate the direct network connections it is involved in (click the blank space to reset).
Supplementary Figure 4.10.: Topographical differences between Wt and Mecp2+/- resting state networks
Interactive map of topographical differences between Wt and Mecp2+/- resting state networks at 16, 20 and 24 weeks old. Each square in this map represents a functional connection between two areas. Using the slider on the right hand side will toggle different age points. Blue squares indicate connections present only in the modelled networks of Wt mice (i.e. absent in Mecp2+/-), while red squares indicate connections found only in Mecp2+/- networks. White squares indicate no difference. Hovering above a square in this plot will reveal the full name of the areas in the pair.